D-Aspartic Acid & Other D-Form Aminos

Nutrition
121

The role and molecular mechanism of D-aspartic acid in the release and synthesis of LH and testosterone in humans and rats.
Topo E, Soricelli A, D’Aniello A, Ronsini S, D’Aniello G.
Source1Stazione Zoologica Anton Dohrn, 80121, Villa Comunale, 80121, Napoli, Italy. enza.topo@szn.it

Abstract
BACKGROUND: D-aspartic acid is an amino acid present in neuroendocrine tissues of invertebrates and vertebrates, including rats and humans. Here we investigated the effect of this amino acid on the release of LH and testosterone in the serum of humans and rats. Furthermore, we investigated the role of D-aspartate in the synthesis of LH and testosterone in the pituitary and testes of rats, and the molecular mechanisms by which this amino acid triggers its action.

METHODS: For humans: A group of 23 men were given a daily dose of D-aspartate (DADAVIT) for 12 days, whereas another group of 20 men were given a placebo. For rats: A group of 10 rats drank a solution of either 20 mM D-aspartate or a placebo for 12 days. Then LH and testosterone accumulation was determined in the serum and D-aspartate accumulation in tissues. The effects of D-aspartate on the synthesis of LH and testosterone were gauged on isolated rat pituitary and Leydig cells. Tissues were incubated with D-aspartate, and then the concentration (synthesis) of LH and cGMP in the pituitary and of testosterone and cAMP in the Leydig cells was determined.

RESULTS: In humans and rats, sodium D-aspartate induces an enhancement of LH and testosterone release. In the rat pituitary, sodium D-aspartate increases the release and synthesis of LH through the involvement of cGMP as a second messenger, whereas in rat testis Leydig cells, it increases the synthesis and release of testosterone and cAMP is implicated as second messenger. In the pituitary and in testes D-Asp is synthesized by a D-aspartate racemase which convert L-Asp into D-Asp. The pituitary and testes possesses a high capacity to trapping circulating D-Asp from hexogen or endogen sources.

CONCLUSION: D-aspartic acid is a physiological amino acid occurring principally in the pituitary gland and testes and has a role in the regulation of the release and synthesis of LH and testosterone in humans and rats.

122

shit you guys just reminded me of my PrimaForce DAA in the cabinet. Noice.

123

OK so this might be placebo effect or something, but I took my first scoop today, and my penis has grown 2 inches.

Just kidding, but my mood was (and is) seriously happy today. I just talked to my opthamologist for like 45 minutes on the eyes, and enjoyed it. Yeah…

124

PB you didnt mix your cocaine and DAA AGAIN did you???
So whats the vote folks? Product sucks or no?

125

[quote]hipsr4runnin wrote:
PB you didnt mix your cocaine and DAA AGAIN did you???
So whats the vote folks? Product sucks or no?[/quote]

Product works for short period of time, then sucks.

126

[quote]PB Andy wrote:
OK so this might be placebo effect or something, but I took my first scoop today, and my penis has grown 2 inches.

Just kidding, but my mood was (and is) seriously happy today. I just talked to my opthamologist for like 45 minutes on the eyes, and enjoyed it. Yeah…[/quote]

PB - did the effects last after the first week or two?

127

[quote]donkeykongJr wrote:

[quote]hipsr4runnin wrote:
PB you didnt mix your cocaine and DAA AGAIN did you???
So whats the vote folks? Product sucks or no?[/quote]

Product works for short period of time, then sucks.[/quote]

agreed, after running it 3 times. first 5 days, awesome. after that, it stops doing anything and makes me feel worse.

128

[quote]wannabebig25 wrote:

[quote]donkeykongJr wrote:

[quote]hipsr4runnin wrote:
PB you didnt mix your cocaine and DAA AGAIN did you???
So whats the vote folks? Product sucks or no?[/quote]

Product works for short period of time, then sucks.[/quote]

agreed, after running it 3 times. first 5 days, awesome. after that, it stops doing anything and makes me feel worse.[/quote]

that’s odd. even with the stuff you added in for estrogen/prolactin control?

129

[quote]MAF14 wrote:

[quote]wannabebig25 wrote:

[quote]donkeykongJr wrote:

[quote]hipsr4runnin wrote:
PB you didnt mix your cocaine and DAA AGAIN did you???
So whats the vote folks? Product sucks or no?[/quote]

Product works for short period of time, then sucks.[/quote]

agreed, after running it 3 times. first 5 days, awesome. after that, it stops doing anything and makes me feel worse.[/quote]

that’s odd. even with the stuff you added in for estrogen/prolactin control?[/quote]

yup. triazole AND dim for estrogen, with mucuna for prolactin. i cant figure it out. all i know is nothing beats tribulus and tongkat lol.

130

Updates on this from those who have taken it? I’m late to the DAA party… and with supplements that is usually a good thing. Results? Any brands to stay away from? Anyone try Way’s crazy stack?

131

[quote]DJS wrote:
Updates on this from those who have taken it? I’m late to the DAA party… and with supplements that is usually a good thing. Results? Any brands to stay away from? Anyone try Way’s crazy stack? [/quote]

4th time around, fantastic results. now i do 3g DAA in the morning with 100mg DIM, and 400mg mucuna pruriens. then at night i do another 100mg DIM and 400mg mucuna. no more nasty estrogen or prolactin sides, and strength gains are awesome.

132

[quote]wannabebig25 wrote:

[quote]DJS wrote:
Updates on this from those who have taken it? I’m late to the DAA party… and with supplements that is usually a good thing. Results? Any brands to stay away from? Anyone try Way’s crazy stack? [/quote]

4th time around, fantastic results. now i do 3g DAA in the morning with 100mg DIM, and 400mg mucuna pruriens. then at night i do another 100mg DIM and 400mg mucuna. no more nasty estrogen or prolactin sides, and strength gains are awesome.[/quote]

Very interesting. Thank you.

133

So, to provide some information, I started using DAA first week April this year.

Training exp to commencing DAA: 3-years (early 30’s age)

Saw no need to cycle, and very much believe that a little bit more than ‘12-days’ is needed to have an idea about it. Initially taking 3g per day in the morning.

Have thoroughly enjoyed using it.

However, of interest, in last 2-3 weeks started taking 2g morning, 2g pre-workout. Noticed a sudden jump in training intensity/volume etc (although bear in mind recently switched up workout drink as well to 30gBCAA, 30g Dextrose + creatine/beta/citruline). Of greater interest was the recent uncomfortable sensation of itchy nipples. Persisted for a couple of days in a row, purchased a commercially available AI and sensation rapidly diminished.

Training gains recently have been extremely positive.

Libido throughout use has not been affected; morning wood has been strong more recently since going to morning/evening split.

Bodyweight change since commencing DAA has been good, but consistent with gradual increase in BW over last year and half.

No bloodwork to provide.

Will continue use as is (2g morning and night).

Recommend that if people want to try it, give it time to work (at least a month). Obviously individual responsiveness is an issue. For other context, I have tried a herbal based t-supp before to no obvious benefit (esp as compared to DAA), and no previous history of other supplementation aimed to increase T levels.

134

hate to bump an old thread but im starting DAA up again in the next couple of weeks and was wondering who else is still on it?

my normal stack of tribulus and tongkat ali has stopped working, and ive read that DIM can actually decrease androgens in males. so after reading all my posts on my DAA experiences ive posted up on here, it seems it works well for myself (and some others) for the first week or so and then stops working. my guess is estrogen starts to creep up and undermine the increase in testosterone.

my 5th time around on DAA, ive prepared by ordering “PES Erase”, a rather strong OTC aromatase inhibitor. i plan on using it for 1-2 weeks before starting the DAA to get estrogen completely under control. i will also grab some P5P in case prolactin increases, as mucuna puriens never did much after a week…

if anybody is interested i can post some updates on here every few days on how well this stack works.

135

Sounds good. What was Way’s crazy stack?

136

[quote]solidkhalid wrote:
Sounds good. What was Way’s crazy stack?[/quote]

[quote]waylanderxx wrote:
I’ve been doing some research on this myself as a buddy tipped me off to this. The NMDA version is vastly superior and more potent to straight up DAA.

Here’s some info from a buddy of mine, take it or leave it:

"DAA’s putative mechanism of action is bioconversion into NMDA and subsequent binding to NMDA receptor sites in neuroendocrine tissue (pituitary, hypothalamus, thalamus etc.) Activation of the NMDA receptors is what we’re looking for and NMDA (N-methyl-D-aspartate) is more potent that DAA by orders of magnitude.

So we … See Morewant DAA to become NMDA as quickly and as fully as it can. The only difference is the methyl group on the Nitrogen, where does the body get that single carbon from? Methyl donors. SAmE and TMG are two potent methyl donors, but there are many. Methylcobalamin (methyl b12) is another. This is addition to the positive health benefits that methyl donors already possess.

There is little to no direct research to support this theory, only educated guesswork, most DAA studies have been done in animal models and NMDA is sort of avoided because of the neurotoxicity buzz.

Verdict: Take DAA with a methyl donor, because it should aid in the conversion to NMDA and hence greater Test/GH increases."

I will be using DAA with a methyl donor. [/quote]

[quote]waylanderxx wrote:
A list of methyl donors:

  1. TMG (betaine)
  2. DMG
  3. Folate… See More
  4. Methylcobalamin
  5. Choline

Take 1-3g of TMG a day along with 3g a day of the DAA. DAA also had wider implications for AAS users and PCT which is where a lot of the excitement is coming from.

I can’t take credit for any of this info here though, I was just tipped off about it today.

WRT: No you won’t need an AI[/quote]

this what you mean?

137

[quote]wannabebig25 wrote:
if anybody is interested i can post some updates on here every few days on how well this stack works.[/quote]

Yeah, would be good to hear about it. I still have a load and may start using it more frequently (3g upon waking) now I’m getting to be more injury-free and able to train more regularly.

I am a bit surprised this stuff hasn’t been highlighted as the natural holy grail it appeared to be when it first came to the fore. Then even Dr Tim on here was raving about its potential. Well, maybe one day…

138

[quote]JamesBrawn007 wrote:

[quote]wannabebig25 wrote:
if anybody is interested i can post some updates on here every few days on how well this stack works.[/quote]

Yeah, would be good to hear about it. I still have a load and may start using it more frequently (3g upon waking) now I’m getting to be more injury-free and able to train more regularly.

I am a bit surprised this stuff hasn’t been highlighted as the natural holy grail it appeared to be when it first came to the fore. Then even Dr Tim on here was raving about its potential. Well, maybe one day…[/quote]

well i just took my first pill of the strongest OTC aromatase inhibitor they make. i need to get my estrogen under control before i go on the DAA. im thinking within the next 3-4 days ill start.

im thinking im gonna do all 3g of DAA pre workout. on non workout days ill do 1.5g in the morning and 1.5g 8 hours later, so the timing syncs up with my AI. im also adding nettle root to increase free testosterone and lower SHBG.

139

[quote]wannabebig25 wrote:

[quote]JamesBrawn007 wrote:

[quote]wannabebig25 wrote:
if anybody is interested i can post some updates on here every few days on how well this stack works.[/quote]

Yeah, would be good to hear about it. I still have a load and may start using it more frequently (3g upon waking) now I’m getting to be more injury-free and able to train more regularly.

I am a bit surprised this stuff hasn’t been highlighted as the natural holy grail it appeared to be when it first came to the fore. Then even Dr Tim on here was raving about its potential. Well, maybe one day…[/quote]

well i just took my first pill of the strongest OTC aromatase inhibitor they make. i need to get my estrogen under control before i go on the DAA. im thinking within the next 3-4 days ill start.

im thinking im gonna do all 3g of DAA pre workout. on non workout days ill do 1.5g in the morning and 1.5g 8 hours later, so the timing syncs up with my AI. im also adding nettle root to increase free testosterone and lower SHBG.[/quote]

how are the results so far?

140

Here’s the stack I’m thinking. Just not sure of the amounts or timing. It’ll be a month or more before I start this. In no particular order and subject to change: Methyl donor, nettle root, mucuna puriens, Rezv, DAA, Zinc. For what it’s worth, I am pretty sensitive to E2 sides. Could be a zinc deficiency? I do have others signs of zinc issues such as white spots on finger nails. Ideally I’d like to get on RezV for a few weeks before I get into the DAA. I’d also like to get BF closer to 12% before I start. I’d guess I’m at about 16% at the moment @ 200 pounds.

On a related note, has anyone in this thread ever considered staking this with Cissus Quadrangularis? I’ve done a good amount of research on this supplement (and have taken it for joints) finding that it has effects on growth hormone (in bone at least), and anecdotally is anabolic at ~2.5 grams daily. Stacking this with the above and carbs periworkout for the ultimate trifecta of anabolic hormones!