Josht9210's TRT Log - Depersonalization, Panic Disorder, Agoraphobia, Etc

[johann77]

@enackers

A measurement is just that. It doesn’t change. The only thing that changes is how much can be measured. So if it’s more accurate and 10 ng/dL is measured in 2019, that same vial might be measured again and it shows 8. But 10 is not 8.

I used to teach biochemistry and genetic classes at the university, so lets try together to solve this puzzle for you. Just be open minded.

Lets take the example from the below link. Its T3, but its representative for T or E2 or any other analyte.

Take a look at the graph (D). You see T3 levels on the x axis (mean and standard deviation) and different assays at the y axis. The exact same samples were measured with 12 different assays (A to M).

Compare for example the results from assay K with the results from assay N. Both assays have compare precision (comparable standard deviation) but results of assay K are on average 25% higher than results from assay N.

Question of the day: How can the results be so different?

Different Reference Ranges for Different Methods

Quite frequently it is discussed here why different labs/assays have different reference ranges. Trying to provide some background why the comparison of lab values is pointless without considering the individual method specific reference range. Lets take T3 as an example: Basically two different assay types exist (also true for T for example): the immunoassay type (e.g. RIA, ECLIA) and the LC-MS based assay LC-MS is considered the golden standard nowadays as it provides the highest specifici…

You may find the video helpful to answer the question

Calibration Methods