TRT Peak reading

I have been on TRT for 12 weeks, at a weekly dose of Test Cyp 100 mg split as Saturday 50mg and Wednesday 50mg. Test Cyp 200mg / ml. Using a 1 ml syringe 0.25ml for 50mg so my dose is accurate.

Cholesterol and blood markers are all in the normal range. Blood pressure is normal 119/73

Recent blood test, blood drawn on Monday ( last TRT shot Saturday)
has total Testosterone at 99.7nmol/L

This is way higher than I was expecting, though I’m guessing I should have drawn blood sample 1 day before my next TRT shot ( Tuesday)

As a peak total Testosterone value this seems very high?

I was expecting to be around 30nmol/L ?

Could I be a hyper responsive to Test Cyp ?

Reduce dose to 80 mg and test blood in next 6 weeks ? This time draw blood day before next trt shot ?

Splitting doses will run your number higher bc the test will not be able to metabolize as completely before you take another shot, compounding if you will. In essence, a little goes further when split. I ran into this same problem and this is how the Dr explained it to me


That’s interesting, then taking once weekly shot of 100mg would result in lower value of total testosterone than splitting total dose in half ?

That’s unfortunate. High cholesterol is healthier. People eating the western diet, reducing red meat consumption has created a new normal. The old normal had high cholesterol the norm and lower disease rates. I wouldn’t listen to mainstream doctors on matters of cholesterol, they’ve all been duped by the pharmaceutical companies propaganda.

It’s not normal to be testing peak levels on TRT. It’s pointless to measure your peak testosterone levels because it is very short-lived! You would be better served testing at midpoint and trough.

This is what happens when you take biotin supplements, it interferes with some methods of lab testing and gives you erroneously high hormone values.

I wouldn’t change your TRT protocol until you’ve been off the biotin supplements for more than a couple of weeks.

The problem is that many common blood tests employ a biotin-streptavidin reaction as part of the test procedure. While the amount of usual dietary biotin intake is not expected to be high enough to affect these tests, biotin supplementation at doses greater than 1 mg per day can cause either falsely low or falsely high test results, depending on the analyte and platform used for testing.

Very interesting @systemlord weirdly I have been supplimenting with B complex and also use Niacin at 100mg for four consecutive days ( Stamets Stack Microdose of Psilocybin ( Truffles 1.2 gram and Lions Main ). Though biotin nothing near 1 mg. Also the day before drawing blood I ate around 200 grams of liver and I also consume around 4-5 eggs per day and eats oatmeal in morning, so possibly this could have pushed up the biotin to >= 1mg

Also supplement:
Vitamin E and D
Omega 3 ( 3000mg )
Vitimin C 2000 mg
Ashwaghanda 800 mg
Calm Magnesium Powder.
Turkey Tail 800mg

Here are the blood results:

I would ignore Total T values going forward due to your insanely high SHBG. Focus solely on the Free T value.

As far as supplementation goes, it’s less about the dose and more about the response to the said dose. I can barely handle a vitamin D level in the mid 30 range. I get toxicity approaching upper 30 ranges.


That’s right, regarding totalTestosterone. The high SHBG is what will affect free testosterone.

So possibly my response to biotin could have skewed my results. I do eat a lot of foods that have biotin. Nuts, Eggs, Yogurt, Avocado…

@systemlord would biotin have an effect on the other markers on the blood results, does biotin interfere mainly with the Hormones?

Second blood results, both venous tests; Medichecks and Optimale done morning at 5:45. Results more in line with what I was expecting.

Results would confirm that my inadvertent consumption of biotin from B complex supplement had skewed results.



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