ECLIA E2 Results Converted to Sensitive

Pharma / TRT Testosterone Replacement
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I read that the ECLIA E2 test results are higher than the sensitive, maybe twice as high. I did a search just now and couldnt find where I read that or anything on that. IM curious what the experienced here think about that.

I started at 390 tt and now trough is 990 on 180mg a week cypionate, 500 hcg and mostly due to this forum and Excel male videos I decided to not take the Ai I was given until seeing how my body would react to adding testosterone.

My E2 was 8 using the ECLIA test as thats what they use and I thought from reading, that that could be as low as 4 if it were the sensitive test. Another reaason I just decided to forego it mostly. I did take 3 doses of .25 at times I worried or felt weird, maybe stupid but whatever.

My E2 ECLIA came back at 36.4 today and while that is not what those here say is optimal, seems like an ok # with 990 TT and maybe could even be lower 20’s, perhaps meaning I should continue not taking my Ai. The ECLIA test says ranges are up to 42.6

My SHBG was 30.3 and is now 30 , so Im not low or high, it seems.

My free test went from 8.27 to to what the doc said today was 30, yet I have no Albumin test so cant equate that myself.

MY T4 was 1.48, TSH was 1.53 and LH was 4.5.

Everything else was in normal range according to labs.

My main question is about how the E2 tests may convert and I will eventually order a sensitive test on my own but am due back in 9 weeks for more bloods so I would like to continue doing what they test for now.

My 36.4 ECLIA seems like it would be mid- low 20’s sensitive by what i recall seeing.

After reading a ton, I was really surprised that I had almost 400TT when tested as I have a history of drug and alcohol abuse along with health food and exercise. I lifted and cycled (bicycle not steriod) hard as a vegan for 12 years when younger, then went fat shit, bought a house, built a business, “no time to exercise”, drink for sorrow, what happened to my penis not working well, etc.

I had a lot of fat around the middle and was pretty weak, Always depressed, yet I had quit drinking almost a year ago and take no other drugs. All helped by taking kratom, which some no less will have an opinion on but has been an amazing wonder for a depressed, tired drug and alcohol addict.

Within a month, I had become another person, I read this is rare and maybe because I already have a good diet, dont do much bad and my work is getting to early partial retirement. I work a couple hours a day now in my early 40’s and will turn it back up after a rest this year to try and push for super early real retirement.

Second month is off the charts for me, sex everyday with my 17 year wife, sometimes twice and throw in a some masturbation. Morning wood just starting to happen periodically. My lifting is back almost to where I was 10 years ago. I got to the point where I was really strong for me and was starting to feel it in my joints, especially both elbows. I think that may have lead to less working out, also getting on xanax for real anxiety. that stuff sucks for being motivated.

Im waiting for the bad sides and not expecting an uptrend for ever but hoping gains in all areas continue like this for a while.

Oh and also my hct was 42 before, I gave blood once and its 44.5 or something like that. It was almost 50 if your hemoglobin multiplied is a proper way to estimate it. So it went up. My doc says donate every couple months. Thankfully I have a red cross in walking distance and I guess it helps people, if its not just going to vampires, which is ok with me too.

I hope this isnt too rambly as thats how my brain works and not so good at putting the thoughts back in order.

Any advice is welcome too. Ive read every forum daily since before I started and lots of issue sto happen I know so just trying to learn as much theory/science as I can in order to keep myself on a decent road.

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Ran both tests concurrently.

LC%20v%20IA%2063%2049
LC%20v%20IA%2063%20493264Ă—1333 677 KB

Go with how you feel. If you feel good at 36.4, don’t worry about bringing it down to the low 20s, regardless of the test.

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Thanks for the screen.

Is the ultra sensitive the sensitive everyone talks about at the top and the bottom the ECLIA?

If thats the case, then its opposite of what I read before that the sensitive will be lower. Or perhaps there is just a huge margin of error?

I agree with feeling good. I just want to air on the side of safety in the E2 turf. I just want to know where I’m truly at for future reference or if I start feeling bad.

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Correct. Here is another one:

IA%20vs%20LCMS%20result
IA%20vs%20LCMS%20result1209Ă—723 157 KB

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Like was stated already. “how do you feel” is 10x more important than numbers.

If you feel “good” and have no nipple itchiness, or a limp dick, don’t worry about your e2.

OH and FOR SURE do not get hung up on a specific “number” that is straight up bullshit.

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Totally understand that. Im just at the begining and want to learn as much as possible.

No nipple weirdness or water retention or any of the described symptoms so I think Im fine. I have to start seeing how I feel after a shot. My trough was 990t, 24 free and 36 ECLIA estrogen. Even high that may be a good ratio.

I get tempted to take a .25 anastrozole when i feel a little weird but sometimes iIdrink too much caffeine, kava and/ or kratom which together can make you not sure what your feeling what from.

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In theory, the numbers should be identical.

The immunoassay (ECLIA) test is more consistent with less chance of operator error. The LC/MS/MS (sensitive) is a more complicated, higher tech, procedure and there is a greater chance of varying results, compared to the IA test.

The IA will not pick up E2 levels below 15 (at least with Quest), so if levels are suspected to be below 15, you’d definitely want the LC/MS test. Otherwise, you get a result of <15.

The immunoassay test can pick up other steroid metabolites, which would yield an artificially high E2 level. The LC/MS will not.

Bottom line, there are pros and cons to each. I would say stick with one and determine what E2 levels work best for you. I ask for both just out of curiosity.

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Here is another one, LC/MS/MS 27, ECLIA 28.

LC%20small%20file

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Thanks again.

So if that is consistent and I’m remembering backwards that would mean my E2 could be higher than 36 on the sensitive.

Still all perhaps good on the 900t , 24 free, 36E2 ratio wise. Perhaps too high of an E2 if I had 400 T still?

Any idea how far trough ranges for people. if Im 990 right before my shot, That could mean I would be at 1250 or some such level at peak Im assuming. I’ll have to search that.

I’ll probably have questions for every answer so if its annoying, I understand.

I find reading about TRT almost as good as doing it. I spent hours yesterday reading and it appears that happening today too. I always get obsessed with new things and it fades after Ive learned or experienced a lot.

Thanks again.

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With your last screen, its just inconsistency so perhaps what I saw before was that sensitive was lower. I asked my doc and he agreed it may be lower than the ECLIA.

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Test would be higher than 1250 right after the injection. As with mine, your sensitive E2 could be higher than the IA, but maybe not. You’d need to run them together to be sure.

If you look at my three labs, IA E2 at 18, 28 and 49. LC/MS 21, 27 and 63. I feel no difference with any of them. I continue to feel great. That’s me. Some guys report E2 symptoms when theirs hit 30. Part of the challenge is determining what protocol works best for you.

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Sorry, it looks clear on my screen, though the last one less so.

The results were:
LC/MS 63, IA 49
LC/MS 21, IA 18
LC/MS 27, IA 28

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Your E2 results are in ascending order by date it looks like. So youve gone up this year but feel no difference? At least from elevated E2. Im guessing you feel a difference from the t.

Did your TT and Ft go up with those E2 #'s every test in a similar % ratio. I can only see the 1028. Have you tested your peak or have estimate of knowing how much it falls every day after a shot?

Amy I ask how long youve been on for those levels?

Told you I would ask a lot of questions but damn is there a lot of crazy new info. I sometimes get to read a multi year thread of someones journey but usually its about side effects and not posting results.

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The latest test with the higher numbers is because I stopped anastrozole for three weeks. I did so because I wanted to see how I would feel at higher E2 levels and I also wanted to see how the two tests would be with higher levels.

My test numbers are usually around 900 total and 200 free, been on TRT for over four years. I’ve always had blood drawn at trough, reportedly, guys on a weekly injection schedule drop 100-150 total a day, so I may top out at 1500-1700 the day after injections. Some inject more frequently to maintain steady levels. There are differing opinions regarding that, but I think you go with how you feel. I feel good throughout the week, no crash, so it works for me. Some metabolize test faster and need more frequent injections.

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Your numbers are great. Stay far away from ai for now. I’ve seen plenty of bloodwork just like highpull’s but in every instance the eclia method was higher. Some times twice as high but not always. It’s my understanding that the eclia method can not differentiate between estradiol and crp (c reactive proteins). C Reactive Proteins are associated with inflammation in the body. That leads me to believe that if you have inflammation than the eclia method can overstate sometime drastically. It’s going to vary from one person to another. Sensitive testing from what I have seen seems to be more consistent and accurate. Regardless, at 36 estradiol you have no worries. Stay the course it sounds like your doing well and I wish you the best.

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Thanks, thats good stuff!

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That seems smart to check and see if there is a difference. If anything from reading anecdotals I see theyre extremely broad. So youre doing once a week shot and see no difference in how you feel. I wonder if after a certain level of TT if the drop isnt as dramatic as someone peaking at 900 and ending up at 500 trough? I would guess.

Is you free T# a different method of measuring than mine? Mine being at 24 and yours being 200 seems like different measurements. Im still getting the measurements a bit confused and have been confused when someone reports their tt at double digit numbers but being good.

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Most assuredly, mine is pg/mL.

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Highpull, One more question for you:)

I figured out how to convert the E2 measurements I think. I would read someones measurements who used a different measurement and it would throw me off.

Your 200 FT is pg/ml which according to the calculator covered to pmol/L = 734 but if its 200pmol/L then your free T would be 54pg/ml which seems to make more sense and still is a really high number of free test I think.

Do you have those backwards or am I still using the wrong measurements?

And if your free T is fact 54, is that tremendously high number or is that just par for the course?

Ive been searching here for someone touching on conversions and also on google which just sends me to medical papers that report things using those measurements. Its getting confusing reading reports and people change how the measure their numbers within the same thread sometimes.

The only thing Ive read that makes sense so far is

I started at 111pmol/L E2 = approximately 30pg/ml E2
Now at 149pmol/L E2 = approximately 40.5pg/ml E2

Its these basic things in the beginning that make everything forward make sense or is there consistency in how people measure their E2 and TT?

Thanks

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Quest reports free test in pg/mL, on the one you can see, it is 256.1. Converting that to ng/dL equals 25.6. With a total test of 1028, that means about 2.5% of my test is free, not a bad number. Between 2-3% is considered good. This is where SHBG comes in, mine hovers at the mid 20s.

The various labs use different measures and have different ranges.

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