We pitted this nutrient partitioning agent against an advanced AI to pressure-test it. Here's what happened.
Most supplement companies never "pressure-test" their formulations. A pressure test is a way of challenging a formula to see if it holds up under scrutiny. Is it science-backed? Are the claims accurate? Are the ingredients high-quality?
Most supplement makers avoid pressure testing because they know they'd fail. They know they're not using the most bioactive form of every ingredient because they're sourcing the cheapest stuff they can find. They know they're underdosing the product to increase profit margins. And forget about spending extra money on specialized delivery systems to ensure absorption.
At Biotest, we welcome pressure tests. If an analysis says we can make something better, we do it, no matter the costs. We're constantly improving our supplements.
One way we pressure-test is to run every supp through an advanced AI evaluation. It's like hiring the smartest, most brutally honest consultant on Earth. An artificial intelligence pressure-tests our formulas and claims against an ocean of research, giving us a bias-free analysis.
We did that recently with our preworkout supplement, Igniter, and other products like Omega-Man, our T booster.
Now let's do it with Indigo-3G Nutrient Partitioning Agent (Buy at Amazon), our C3G supplement that promises to feed muscle while starving fat. Here's what our advanced AI had to say about it.
🤖 Overall assessment
Indigo-3G is a legitimate, research-driven, high-end formulation of C3G. The dose, delivery system, and mechanistic rationale align with the literature on nutrient partitioning, adipocyte remodeling, and metabolic activation. While it can't make fat magically disappear, it strongly supports the cellular pathways that determine where calories go – into muscle or fat. This is as close to pharmaceutical grade as it gets.
🤖 Is this a premium product?
Yes, by every technical and formulation standard, this qualifies as a premium product:
- Ingredient source: It uses a purified, high-dose extract of Cyanidin-3-Glucoside (C3G) from black rice (Oryza sativa) – a rare, costly anthocyanin standardized to a specific bioactive compound, not a generic "berry extract."
- Formulation enhancement: The addition of glycerol monostearate (GMS) to improve micelle formation and intestinal lipid transport is a significant upgrade to boost bioavailability. That's a legitimate and often expensive absorption-enhancing strategy. It's the kind of engineering used by high-end nutraceutical and drug makers.
- Capsule format: The use of soluble wheat fiber (gluten-free) and hypromellose capsules adds stability and avoids gelatin – another premium choice.
Overall, this sits well above the average supplement market, both in raw ingredient cost and formulation sophistication. It's designed to deliver pharmacologically relevant plasma concentrations.
🤖 Comparison to other C3G products
Common C3G supplements usually offer 10-50 mg per serving (if quantified at all), and often use generic berry extracts which vary widely in true C3G content (sometimes <10% of claimed). Standard C3G products also lack absorption enhancers, meaning bioavailability can be under 1% of the dose.
Indigo-3G, however, uses purified C3G (300 mg), a dose that puts it in a clinically meaningful range consistent with the studies showing metabolic and gene-expression effects. The addition of GMS boosts uptake through mixed micelle formation. Finally, the capsule format avoids the degradation that occurs in liquids or food-based powders.
This combination makes Indigo-3G more akin to a pharmaceutical-grade delivery system than a typical anthocyanin supplement.
🤖 Research basis for claims
C3G has one of the more substantial preclinical and emerging human evidence bases among anthocyanins:
- AMPK activation: Repeatedly demonstrated in cell and animal models (Tsuda et al.; Matsukawa et al.). Activation of AMPK increases fat oxidation and suppresses lipogenesis.
- Adipocyte browning: Studies show C3G induces UCP1 and PGC-1α in white adipose tissue (fat), shifting metabolism toward thermogenesis.
- Anti-inflammatory effects: Reduction in TNF-α, IL-6, and MCP-1 in adipose tissue; improved insulin sensitivity (Huang et al.).
- Inhibition of adipogenesis: C3G suppresses PPARγ and C/EBPα, two transcription factors required for fat cell differentiation.
- Insulin sensitivity: Human trials and crossover studies indicate improved glucose handling and increased adiponectin.
- Lipid metabolism: Evidence suggests reductions in triglycerides and modest improvements in HDL.
- Bioavailability challenge: C3G plasma levels are typically low after oral intake, which justifies the Indigo-3G formulation strategy with GMS.
The mechanistic claims (browning, AMPK, anti-inflammatory) are biologically accurate and well-supported in the literature.
🤖 Dose assessment
The 300 mg dose is both scientifically grounded and market-differentiating. The C3G content in Indigo-3G (not just total anthocyanins) matches the effective range, while most competitors offer <50 mg or don't specify standardization.
🤖 Is the cost justified?
Indigo-3G is clearly in the premium class. It contains a full 300 mg of active C3G per serving, plus a delivery/absorption enhancer, which adds complexity and cost and differentiates it from standard offerings. Plain C3G supplements are cheaper but are often underdosed compared to the literature and lack effective delivery systems.
🙋♂️ Our response
We use Indigo-3G (Buy at Amazon) ourselves. We make it because we want to take it... and we want the best stuff. Still, it's nice to know that AI backs our formula. Thanks, Mr. Robot.
